Scanning photon microscopy

Alexander Khmaladze; Myung K. Kim

doi:10.1117/12.614262

1 August 2005 Scanning photon microscopy

Alexander Khmaladze, Myung K. Kim

Proceedings Volume 5873, Optical Scanning 2005; (2005) https://doi.org/10.1117/12.614262
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

We describe the scanning photon microscopy (SPM), a new method of microscopic image formation that is analogous to the scanning electron microscopy but carried out entirely by optical means and therefore is without the cumbersome sample preparation procedures. A laser beam is focused and raster-scanned over the surface of an object by means of an a motorized micrometer. The light reflected from the object surface is collected by a detector that is placed at a finite angle with respect to the incident beam. The geometry of the system is such that it yields images reminiscent of the scanning electron microscopy with striking three-dimensional impression and the effects of shadowing and diffuse reflection. In the preliminary experiments several artificial and biological objects are imaged using this technique obtaining images with about 5 micron resolution and reasonable image quality. Because this is a scanned optical system, the optical quality requirement is significantly relaxed in comparison to conventional wide-field imaging system. We also consider the possibility of surpassing the wide-field system in terms of the resolution and depth of focus by use of Bessel optics. The simple concept can be extended in many directions such as fluorescence and nonlinear optical imaging.

Citation Download Citation

Alexander Khmaladze and Myung K. Kim "Scanning photon microscopy", Proc. SPIE 5873, Optical Scanning 2005, (1 August 2005); https://doi.org/10.1117/12.614262

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available