Immobilized gold nanoparticles fabricated by template assisted organo metallic chemical vapor deposition for oligonucleotide hybridisaton studies

Anne Kathrena A. Aliganga; Fei Xu; Wolfgang Knoll; Silvia Mittler

doi:10.1117/12.629158

12 October 2005 Immobilized gold nanoparticles fabricated by template assisted organo metallic chemical vapor deposition for oligonucleotide hybridisaton studies

Anne Kathrena A. Aliganga, Fei Xu, Wolfgang Knoll, Silvia Mittler

Author Affiliations +

Proceedings Volume 5969, Photonic Applications in Biosensing and Imaging; 59690H (2005) https://doi.org/10.1117/12.629158
Event: Photonics North, 2005, Toronto, Canada

Abstract

Immobilized gold nanoparticles on oxide surfaces were prepared by template assisted organometallic chemical vapor deposition (OMCVD). The growth parameters, optical parameters such as the plasmon resonance band, as well as geometrical parameters such as size and shape are investigated by UV-Vis spectroscopy, AFM, TEM and SEM. These new kind of gold nanoparticles were applied on a fluorescence based oligonucleotide hybridization study. Gold nanoparticles with a mean lateral diameter of 12 nm yielding an extinction maximum at around 520-530 nm should be able to resonantly excite the Cy3-fluorescence label attached to the target oligonucleotide. The hybridization reaction is taking place between gold nanoparticle surface-attached oligonucleotide catcher strands and chromophore-labeled target strands in solution. The experiment was conducted in the form of classical total internal reflection fluorescence detection. The kinetic data were quantitatively analyzed following a simple Langmuir model. It was found that a single mismatch between oligonucleotide target and probe reduces the Langmuir equilibrium constant by two orders of magnitude, allowing for an excellent sequence-specific detection of oligonucleotide hybridization based on OMCVD gold nanoparticles.

Citation Download Citation

Anne Kathrena A. Aliganga, Fei Xu, Wolfgang Knoll, and Silvia Mittler "Immobilized gold nanoparticles fabricated by template assisted organo metallic chemical vapor deposition for oligonucleotide hybridisaton studies", Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 59690H (12 October 2005); https://doi.org/10.1117/12.629158

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