Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy

Jihong Zheng; Yanmeng Jiang; Aiqing Huang; Guoqiang Sun; Songlin Zhuang; Shizhuo Yin

doi:10.1117/12.862151

18 August 2010 Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy

Jihong Zheng, Yanmeng Jiang, Aiqing Huang, Guoqiang Sun, Songlin Zhuang, Shizhuo Yin

Author Affiliations +

Proceedings Volume 7781, Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV; 778115 (2010) https://doi.org/10.1117/12.862151
Event: SPIE Photonic Devices + Applications, 2010, San Diego, California, United States

Abstract

A novel two channel multi-frequencies division multiplexed confocal fluorescence microscopy (FDMCF) system based on the holographic polymer dispersed liquid crystal (H-PDLC) optical chopper modulation is reported. The characteristic of unique FDMCF system is that within the FDMCF system, the multi-channel exciter laser beams are modulated with different carrying frequencies, after the fluorescence signals are collected, and through data analyzing process, the FDMCF system can realize the multiply points parallel detection synchronously with high temporal and spatial resolution. Besides, combined with the electrically controlled H-PDLC grating array working as the optical chopper to replace the mechanical chopper, it can improve and develop the FDMCF system from two channels to unlimited system. The paper has done the experiments to demonstrate the two-channel FDMCF system based on H-PDLC modulation and analysis the benefits from the electrically-controlled integrated electro-optical H-PDLC devices.

Citation Download Citation

Jihong Zheng, Yanmeng Jiang, Aiqing Huang, Guoqiang Sun, Songlin Zhuang, and Shizhuo Yin "Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy", Proc. SPIE 7781, Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV, 778115 (18 August 2010); https://doi.org/10.1117/12.862151

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