Investigation of shape memory of red blood cells using optical tweezers and quantitative phase microscopy

Nelson Cardenas; Samarendra K. Mohanty

doi:10.1117/12.909758

13 February 2012 Investigation of shape memory of red blood cells using optical tweezers and quantitative phase microscopy

Nelson Cardenas, Samarendra K. Mohanty

Author Affiliations +

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 82252B (2012) https://doi.org/10.1117/12.909758
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

RBC has been shown to possess shape memory subsequent to shear-induced shape transformation. However, this property of RBC may not be generalized to all kinds of stresses. Here, we report our observation on the action of radiation pressure forces on RBC's shape memory using optical manipulation and quantitative phase microscopy (OMQPM). QPM, based on Mach-Zehnder interferrometry, allowed measurement of dynamic changes of shape of RBC in optical tweezers at different trapping laser powers. In high power near-infrared optical tweezers (>200mW), the RBC was found to deform significantly due to optical forces. Upon removal of the tweezers, hysteresis in recovering its original resting shape was observed. In very high power tweezers or long-term stretching events, shape memory was almost erased. This irreversibility of the deformation may be due to temperature rise or stress-induced phase transformation of lipids in RBC membrane.

Citation Download Citation

Nelson Cardenas and Samarendra K. Mohanty "Investigation of shape memory of red blood cells using optical tweezers and quantitative phase microscopy", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82252B (13 February 2012); https://doi.org/10.1117/12.909758

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