Presentation
7 March 2022 Second harmonic generation imaging of collagen changes in idiopathic pulmonary fibrosis
Paul J. Campagnola, Darian James, Emily Shelton, Nathan Sando, Mark Jones
Author Affiliations +
Abstract
We use SHG microscopy to study collagen alterations in idiopathic pulmonary fibrosis (IPF) in human tissues and in vitro models. Texture analysis successfully classified the fiber morphology in normal and IPF tissues at near 100% accuracy. SHG polarization measurement indicated reduced chirality in the collagen triple helix in IPF in both tissues and spheroid models. Using SHG directional measurements (F/B), we found the collagen fibril and macromolecular structures are altered in IPF, consistent with decreased organization. We are developing a rigorous phasematching approach to determine the collagen fibril size, packing, and polarity based on these measurements.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Paul J. Campagnola, Darian James, Emily Shelton, Nathan Sando, and Mark Jones "Second harmonic generation imaging of collagen changes in idiopathic pulmonary fibrosis", Proc. SPIE PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII, PC119650L (7 March 2022); https://doi.org/10.1117/12.2609830
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KEYWORDS
Collagen

Tissues

Second-harmonic generation

Pulmonary fibrosis

In vitro testing

3D modeling

Modulation

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