Quantifying age-related changes in skin metabolism and collagen organization using in vivo multiphoton microscopy

Marcos Rodriguez; Kyle P. Quinn; Alan E Woessner; Ragan Edison

doi:10.1117/12.2668672

17 March 2023 Quantifying age-related changes in skin metabolism and collagen organization using in vivo multiphoton microscopy

Marcos Rodriguez, Kyle P. Quinn, Alan E Woessner, Ragan Edison

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Proceedings Volume PC12352, Photonics in Dermatology and Plastic Surgery 2023; PC123520M (2023) https://doi.org/10.1117/12.2668672
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Intrinsic aging is associated with metabolic dysregulation and altered tissue organization. Using label-free multiphoton microscopy, skin was non-invasively imaged in vivo in young and aged mice and optical biomarkers of metabolism and altered collagen organization were compared. Aged skin demonstrated decreased NADH autofluorescence intensity, higher mitochondrial fractal dimensions, and longer mean NADH fluorescence lifetimes compared to young skin. Moreover, aged dermal collagen exhibited greater randomness of fiber organization. These findings demonstrate that multiphoton microscopy is a viable technique to monitor multiple structural and biochemical changes associated with the aging process.

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Marcos Rodriguez, Kyle P. Quinn, Alan E Woessner, and Ragan Edison "Quantifying age-related changes in skin metabolism and collagen organization using in vivo multiphoton microscopy", Proc. SPIE PC12352, Photonics in Dermatology and Plastic Surgery 2023, PC123520M (17 March 2023); https://doi.org/10.1117/12.2668672

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KEYWORDS

Skin

Collagen

Multiphoton microscopy

In vivo imaging

Mode conditioning cables

Fractal analysis

Mitochondria

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