Over the last decade, there has been rapid development of mode-locked femtosecond fiber lasers with the clear goal of providing an easy-to-use alternative to older technologies for two-photon microscopy applications. Single-wavelength fiber lasers have the potential to revolutionize two-photon fluorescence microscopy by making simple and cost-efficient light sources available to everyone. With short, clean pulses, these robust, turn-key lasers enable unmatched brightness in two-photon microscopy without unwanted heating of the sample. This technology enables the laser to be used in next-generation mobile applications, such as with the MINI2P microscope for measurement in freely-moving, unrestrained mice. This talk will explore some of these applications.
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