Spatiotemporal optical manipulation of intracellular molecular activities

Bin Dong; Shivam Mahapatra; Chi Zhang; Matthew G. Clark; Karsten Mohn; Seohee Ma; Kent Brasseale

doi:10.1117/12.2692750

13 March 2024 Spatiotemporal optical manipulation of intracellular molecular activities

Bin Dong, Shivam Mahapatra, Chi Zhang, Matthew G. Clark, Karsten Mohn, Seohee Ma, Kent Brasseale

Proceedings Volume PC12840, Optical Interactions with Tissue and Cells XXXV; PC1284001 (2024) https://doi.org/10.1117/12.2692750
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Laser-based optical control technologies have shown promise in achieving high spatiotemporal precision. However, existing methods face challenges in real-time target selection and manipulation. To overcome these limitations, we present a real-time precision opto-control (RPOC) technology, which is a closed-loop optoelectronic system that is built upon a laser scanning confocal fluorescence microscope and integrates chemical-specific optical detection, real-time decision-making, and precise optical manipulation at target sites. Using RPOC, we demonstrated precise inducing reactive oxygen species (ROS) solely at selected targets and monitoring ROS-induced changes in microtubule polymerization dynamics. We also selectively inhibit tubulin polymerization using RPOC paired with a photoswitchable inhibitor.

Conference Presentation

Citation Download Citation

Bin Dong, Shivam Mahapatra, Chi Zhang, Matthew G. Clark, Karsten Mohn, Seohee Ma, and Kent Brasseale "Spatiotemporal optical manipulation of intracellular molecular activities", Proc. SPIE PC12840, Optical Interactions with Tissue and Cells XXXV, PC1284001 (13 March 2024); https://doi.org/10.1117/12.2692750

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