Laser speckle particle sizer (SPARSE) for characterizing cellular structures in tissue

Zeinab Hajjarian Kashany; Seemantini Nadkarni

doi:10.1117/12.3003100

13 March 2024 Laser speckle particle sizer (SPARSE) for characterizing cellular structures in tissue

Zeinab Hajjarian Kashany, Seemantini Nadkarni

Proceedings Volume PC12856, Biomedical Applications of Light Scattering XIV; PC1285604 (2024) https://doi.org/10.1117/12.3003100
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Particle sizing of cellular structures may have significant diagnostic utility. We introduce a laser Speckle PARticle SizEr (SPARSE) that harnesses the spatio-temporal analysis of polarized speckle to estimate scattering particle sizes in opaque biofluids and tissues. SPARSE measurements significantly correlate with dynamic light scattering (DLS) in polybead suspensions (R2=0.91, p<.0001) and milk samples (R2=0.93, p<.0001). Similarly, in whole blood samples of increasing tonicities, SPARSE tracks RBC shrinkage in concordance with DLS (R2=0.84, p<.0001). Moreover, SPARSE maps of breast carcinoma reveal distinct sizes for adipose, fibrous, and epithelial compartments. These findings highlight the diagnostic potential of SPARSE in multiple conditions.

Conference Presentation

Citation Download Citation

Zeinab Hajjarian Kashany and Seemantini Nadkarni "Laser speckle particle sizer (SPARSE) for characterizing cellular structures in tissue", Proc. SPIE PC12856, Biomedical Applications of Light Scattering XIV, PC1285604 (13 March 2024); https://doi.org/10.1117/12.3003100

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