Laser speckle contrast imaging augmented with structured light

Jaime Necochea; Jaime Prieto; Zachary Hoffman; Charles DiMarzio

doi:10.1117/12.2275272

24 February 2017 Laser speckle contrast imaging augmented with structured light

Jaime Necochea, Jaime Prieto, Zachary Hoffman, Charles DiMarzio

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Proceedings Volume 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV; 100701F (2017) https://doi.org/10.1117/12.2275272
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

This paper presents a new method of blood flow imaging in vessels within and underneath the skin. The combination of Laser Speckle Contrast Imaging (LSCI) and Spatial Frequency Domain Imaging (SFDI)/ Structured Illumination Microscopy (SIM) allows one to retrieve a substantial amount of flow information, which is otherwise occluded by the skin. In this article, the experiment consists of illuminating a phantom with coherent light passing through a grid pattern which is modulated at the plane of interest. The speckle pattern is then imaged and processed using a combination of structured illumination sectioning and laser speckle contrast. The whole process is realized using an inexpensive setup consisting of a laser diode (Wavelength = 758 nm), mirrors, lenses, a grid and an 8-bit camera. This advanced, yet easy to implement, technique will allow deep skin imaging to be fast, effective and inexpensive.

Citation Download Citation

Jaime Necochea, Jaime Prieto, Zachary Hoffman, and Charles DiMarzio "Laser speckle contrast imaging augmented with structured light", Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 100701F (24 February 2017); https://doi.org/10.1117/12.2275272

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KEYWORDS

Speckle

Laser speckle contrast imaging

Image processing

Speckle pattern

Demodulation

Skin

Light scattering

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