Inverted Confocal Microscopy For Biological And Material Applications

G. J. Brakenhoff; R W. Wijnaendts-van-Resandt; J. Engelhardt; W. Knebel; H. T. M. van der Voort

doi:10.1117/12.950337

9 February 1989 Inverted Confocal Microscopy For Biological And Material Applications

G. J. Brakenhoff, R W. Wijnaendts-van-Resandt, J. Engelhardt, W. Knebel, H. T. M. van der Voort

Proceedings Volume 1028, Scanning Imaging; (1989) https://doi.org/10.1117/12.950337
Event: 1988 International Congress on Optical Science and Engineering, 1988, Hamburg, Germany

Abstract

Aspects of the inverted versus top view configuration for confocal microscopy are examined with the inverted-type clearly offering advantages for high-resolution imaging of certain types of biological specimen. The role and possibilities of the use of pinholes of variable size in confocal microscopy is discussed and data are presented for combinations of objectives and various sizes of pinholes.

Citation Download Citation

G. J. Brakenhoff, R W. Wijnaendts-van-Resandt, J. Engelhardt, W. Knebel, and H. T. M. van der Voort "Inverted Confocal Microscopy For Biological And Material Applications", Proc. SPIE 1028, Scanning Imaging, (9 February 1989); https://doi.org/10.1117/12.950337

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