Multimodal nonlinear imaging of arabidopsis thaliana root cell

Bumjoon Jang; Sung-Ho Lee; Sooah Woo; Jong-Hyun Park; Myeong Min Lee; Seung-Han Park

doi:10.1117/12.2270697

12 July 2017 Multimodal nonlinear imaging of arabidopsis thaliana root cell

Bumjoon Jang, Sung-Ho Lee, Sooah Woo, Jong-Hyun Park, Myeong Min Lee, Seung-Han Park

Author Affiliations +

Proceedings Volume 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017; 103240C (2017) https://doi.org/10.1117/12.2270697
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 2017, Jeju, Korea, Republic of

Abstract

Nonlinear optical microscopy has enabled the possibility to explore inside the living organisms. It utilizes ultrashort laser pulse with long wavelength (greater than 800nm). Ultrashort pulse produces high peak power to induce nonlinear optical phenomenon such as two-photon excitation fluorescence (TPEF) and harmonic generations in the medium while maintaining relatively low average energy pre area. In plant developmental biology, confocal microscopy is widely used in plant cell imaging after the development of biological fluorescence labels in mid-1990s. However, fluorescence labeling itself affects the sample and the sample deviates from intact condition especially when labelling the entire cell. In this work, we report the dynamic images of Arabidopsis thaliana root cells. This demonstrates the multimodal nonlinear optical microscopy is an effective tool for long-term plant cell imaging.

Citation Download Citation

Bumjoon Jang, Sung-Ho Lee, Sooah Woo, Jong-Hyun Park, Myeong Min Lee, and Seung-Han Park "Multimodal nonlinear imaging of arabidopsis thaliana root cell", Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 103240C (12 July 2017); https://doi.org/10.1117/12.2270697

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KEYWORDS

Luminescence

Multimodal imaging

Microscopy

Optical microscopy

Ultrafast phenomena

Biology

Botany

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