Novel snapshot hyperspectral imager for fluorescence imaging

Lynn Chandler; Andrea Chandler; Ammasi Periasamy

doi:10.1117/12.2300933

23 February 2018 Novel snapshot hyperspectral imager for fluorescence imaging

Lynn Chandler, Andrea Chandler, Ammasi Periasamy

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 1049837 (2018) https://doi.org/10.1117/12.2300933
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Hyperspectral imaging has emerged as a new technique for the identification and classification of biological tissue1. Benefitting recent developments in sensor technology, the new class of hyperspectral imagers can capture entire hypercubes with single shot operation and it shows great potential for real-time imaging in biomedical sciences. This paper explores the use of a SnapShot imager in fluorescence imaging via microscope for the very first time. Utilizing the latest imaging sensor, the Snapshot imager is both compact and attachable via C-mount to any commercially available light microscope. Using this setup, fluorescence hypercubes of several cells were generated, containing both spatial and spectral information. The fluorescence images were acquired with one shot operation for all the emission range from visible to near infrared (VIS-IR). The paper will present the hypercubes obtained images from example tissues (475-630nm). This study demonstrates the potential of application in cell biology or biomedical applications for real time monitoring.

Citation Download Citation

Lynn Chandler, Andrea Chandler, and Ammasi Periasamy "Novel snapshot hyperspectral imager for fluorescence imaging", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049837 (23 February 2018); https://doi.org/10.1117/12.2300933

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KEYWORDS

Imaging systems

Luminescence

Hyperspectral imaging

Microscopes

Near infrared

Visible radiation

Biomedical optics

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