Ultrafast single molecule technique for the study of force dependent kinetics and conformational changes of actin-protein interaction involved in mechanotransduction

M. Sergides; C. Arbore; F. S. Pavone; M. Capitanio

doi:10.1117/12.2288384

20 February 2018 Ultrafast single molecule technique for the study of force dependent kinetics and conformational changes of actin-protein interaction involved in mechanotransduction

M. Sergides, C. Arbore, F. S. Pavone, M. Capitanio

Author Affiliations +

Proceedings Volume 10500, Single Molecule Spectroscopy and Superresolution Imaging XI; 105000S (2018) https://doi.org/10.1117/12.2288384
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Mechanical signals occurring at the interface between cell membrane and extracellular matrix and at intercellular junctions trigger biochemical signals that are fundamental for cell growth, development and regulation. Adaptor proteins, which link the cell membrane to the actin cytoskeleton, seem to partake in this process of mechanotransduction. In particular, catenins play a key role in intercellular junctions, where they act as a bridge between the cell membrane and actin. Studies suggest that α-catenin contains a domain that normally masks vinculin binding sites, which can become accessible after a conformational change induced by an external force. Here we demonstrate a single-molecule technique for investigating actin-protein interactions at different forces (up to ~17 pN) with adequate temporal resolution (sub-ms). This system is based on the ultrafast force-clamp spectroscopy technique that has been recently developed by our group and is adapted to study and measure force-dependent kinetics of the catenin-actin interaction, as well as the amplitude of the expected conformational changes such as force-induced protein unfolding.

Citation Download Citation

M. Sergides, C. Arbore, F. S. Pavone, and M. Capitanio "Ultrafast single molecule technique for the study of force dependent kinetics and conformational changes of actin-protein interaction involved in mechanotransduction", Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000S (20 February 2018); https://doi.org/10.1117/12.2288384

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