Label-free infrared spectroscopic imaging for characterization of necrotic tissue areas on cutaneous squamous cell carcinoma

Cássio A. Lima; Luciana Correa; Hugh J. Byrne; Denise M. Zezell

doi:10.1117/12.2510711

26 February 2019 Label-free infrared spectroscopic imaging for characterization of necrotic tissue areas on cutaneous squamous cell carcinoma

Cássio A. Lima, Luciana Correa, Hugh J. Byrne, Denise M. Zezell

Author Affiliations +

Proceedings Volume 10868, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII; 1086812 (2019) https://doi.org/10.1117/12.2510711
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

In this study, FTIR spectroscopy was used to evaluate the overall biochemical status of necrotic tissue areas of cutaneous squamous cell carcinoma chemically-induced on mice. FTIR hyperspectral image collected from specimen showed high correlation with the photomicrograph obtained by light microscopy, in which we were able to identify clusters associated to keratin, necrosis and regions with no tissue. Alterations in the protein content were documented in the necrotic tissue areas, indicating changes on protein conformation.

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Citation Download Citation

Cássio A. Lima, Luciana Correa, Hugh J. Byrne, and Denise M. Zezell "Label-free infrared spectroscopic imaging for characterization of necrotic tissue areas on cutaneous squamous cell carcinoma", Proc. SPIE 10868, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII, 1086812 (26 February 2019); https://doi.org/10.1117/12.2510711

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