Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

Arnica Karuna; Francesco Masia; Marie Wiltshire; Rachel Errington; Paola Borri; Wolfgang Langbein

doi:10.1117/12.2510277

22 February 2019 Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

Arnica Karuna, Francesco Masia, Marie Wiltshire, Rachel Errington, Paola Borri, Wolfgang Langbein

Author Affiliations +

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108821P (2019) https://doi.org/10.1117/12.2510277
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Quantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.

Citation Download Citation

Arnica Karuna, Francesco Masia, Marie Wiltshire, Rachel Errington, Paola Borri, and Wolfgang Langbein "Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108821P (22 February 2019); https://doi.org/10.1117/12.2510277

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