High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

Stylianos E. Psycharakis; Evangelos Liapis; Athanasios Zacharopoulos; Mariam-Eleni Oraiopoulou; Chrysoula Aivalioti; Vangelis Sakkalis; Joseph Papamatheakis; Jorge Ripoll; Giannis Zacharakis

doi:10.1117/12.2527214

22 July 2019 High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

Stylianos E. Psycharakis, Evangelos Liapis, Athanasios Zacharopoulos, Mariam-Eleni Oraiopoulou, Chrysoula Aivalioti, Vangelis Sakkalis, Joseph Papamatheakis, Jorge Ripoll, Giannis Zacharakis

Author Affiliations +

Proceedings Volume 11076, Advances in Microscopic Imaging II; 110760O (2019) https://doi.org/10.1117/12.2527214
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Breast cancer and Glioblastoma brain cancer are aggressive malignancies with poor prognosis. In this study primary Glioblastoma and secondary breast cancer spheroids are formed and treated with the well-known Temozolomide and Doxorubicin chemotherapeutics, respectively. A custom multi-angle Light Sheet Fluorescence Microscope is employed for high resolution imaging of both cancer cell spheroids. Such a technique is successful in realizing pre-clinical drug screening, while enables the discrimination among physiologic tumor parameters. LSFM technique, parameters and method followed are also presented.

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Stylianos E. Psycharakis, Evangelos Liapis, Athanasios Zacharopoulos, Mariam-Eleni Oraiopoulou, Chrysoula Aivalioti, Vangelis Sakkalis, Joseph Papamatheakis, Jorge Ripoll, and Giannis Zacharakis "High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760O (22 July 2019); https://doi.org/10.1117/12.2527214

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