Quantitative fluorescence spectroscopy to detect and identify bacteria and to monitor their viability (Conference Presentation)

Frédérique Vanholsbeeck; Julia Robertson; Fang Ou; Claire Honney; Rachel Guo; Simon Swift; Cushla M. McGoverin

doi:10.1117/12.2545243

10 March 2020 Quantitative fluorescence spectroscopy to detect and identify bacteria and to monitor their viability (Conference Presentation)

Frédérique Vanholsbeeck, Julia Robertson, Fang Ou, Claire Honney, Rachel Guo, Simon Swift, Cushla M. McGoverin

Author Affiliations +

Proceedings Volume 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020; 1122304 (2020) https://doi.org/10.1117/12.2545243
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Detecting and characterising bacteria as well as monitoring their viability are routine tasks in microbiology. Conventional methods of cell detection and viability monitoring are often time-consuming and/or expensive. We have developed a near-real time, cost-effective and portable fluorometer, the optrode, for quantifying fluorescence signals. We are currently developing protocols to use the optrode to detect, identify and quantify bacteria as well as to monitor their viability using nucleic acid stains such as SYTO 9 and propidium iodide that are routinely used as live/dead stains. Our results are promising but indicate that better dyes are needed to fully characterise bacteria.

Conference Presentation

Citation Download Citation

Frédérique Vanholsbeeck, Julia Robertson, Fang Ou, Claire Honney, Rachel Guo, Simon Swift, and Cushla M. McGoverin "Quantitative fluorescence spectroscopy to detect and identify bacteria and to monitor their viability (Conference Presentation)", Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 1122304 (10 March 2020); https://doi.org/10.1117/12.2545243

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