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9 March 2020 Pathological crystal imaging using computational polarized light microscopy (Conference Presentation)
Bijie Bai, Hongda Wang, Tairan Liu, Yair Rivenson, John Fitzgerald, Aydogan Ozcan
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Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112430F (2020) https://doi.org/10.1117/12.2547403
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
We report a single-shot computational polarized-light microscopy (SCPLM) method for identifying pathological crystals in bodily-fluids. We utilize the four-directional polarizers integrated on the pixels of a CMOS image sensor to reconstruct the transmittance, retardance, and slow-axis orientation maps of the objects with a single image exposure. Using SCPLM, we imaged birefringent crystals found in synovial fluid, e.g., monosodium urate, calcium pyrophosphate dehydrate, and triamcinolone acetonide. The quantitative birefringence images created by our method are pseudo-colored and digitally-integrated with bright-field images to highlight the birefringent crystals within the background. We believe this single-shot, quantitative, and easy-to-operate method will significantly benefit rheumatology.
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Bijie Bai, Hongda Wang, Tairan Liu, Yair Rivenson, John Fitzgerald, and Aydogan Ozcan "Pathological crystal imaging using computational polarized light microscopy (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430F (9 March 2020); https://doi.org/10.1117/12.2547403
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KEYWORDS
Crystals
Microscopy
Polarized microscopy
Birefringence
Polarizers
Microfluidic imaging
Microfluidics
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