Electronically controllable multiple line-scanning confocal microscopy using digital micromirror device (Conference Presentation)

Chun-Hung Weng; Kyu Young Han

doi:10.1117/12.2545072

9 March 2020 Electronically controllable multiple line-scanning confocal microscopy using digital micromirror device (Conference Presentation)

Chun-Hung Weng, Kyu Young Han

Author Affiliations +

Proceedings Volume 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII; 1124513 (2020) https://doi.org/10.1117/12.2545072
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We propose the multiple focused inclined beams in conjunction a highly efficient detection system and electronically controllable confocal slit array in the fluorescence microscope system to obtain low photodamage, good optical sectioning, video-rate, and high-sensitivity imaging. To do so, we use a digital micro-mirror to generate the multiple beams and to form the detection slit synchronously. This provides the high resolution, the capability to image thick sample and the longer observation time while maintaining a similar imaging speed. By directly programming the digital micro-mirror we could correct the anisotropic beam shape and suppress the background.

Conference Presentation

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Chun-Hung Weng and Kyu Young Han "Electronically controllable multiple line-scanning confocal microscopy using digital micromirror device (Conference Presentation)", Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 1124513 (9 March 2020); https://doi.org/10.1117/12.2545072

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KEYWORDS

Confocal microscopy

Digital micromirror devices

Luminescence

Imaging systems

Electronic components

Tissues

Micromirrors

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