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9 March 2020 Achieving spontaneous super-resolution in a confocal microscope by exploiting super-linear emitters (Conference Presentation)
Denitza Denkova, Martin Ploschner, Minakshi Das, Lindsay M. Parker, Xianlin Zheng, Yiqing Lu, Antony Orth, Nicolle H. Packer, James A. Piper
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Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460H (2020) https://doi.org/10.1117/12.2544364
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
We achieve spontaneous 3D super-resolution on a standard confocal microscope by exploiting bio-friendly fluorescent markers with super-linear excitation-emission dependence (upconversion nanoparticles of NaYF4: Yb, Tm). We refer to this approach as upconversion super-linear excitation-emission (uSEE) microscopy. To demonstrate the applicability of the method for biological applications, we image sugar-coated upconversion nanoparticles in neuronal cells and we achieve resolution twice better than the diffraction limit both in lateral and axial directions. We envision that due to the application simplicity of the developed methodological toolbox, uSEE microscopy can be widely incorporated as an everyday super-resolution method in biological laboratories.
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Denitza Denkova, Martin Ploschner, Minakshi Das, Lindsay M. Parker, Xianlin Zheng, Yiqing Lu, Antony Orth, Nicolle H. Packer, and James A. Piper "Achieving spontaneous super-resolution in a confocal microscope by exploiting super-linear emitters (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460H (9 March 2020); https://doi.org/10.1117/12.2544364
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KEYWORDS
Super resolution
Confocal microscopy
Microscopes
3D image processing
Stereoscopy
Microscopy
Nanoparticles
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