The role of photochemical transformations of tetrahydrobiopterin in the pathogenesis and phototherapy of vitiligo

Alexey S. Nizamutdinov; Taisia A. Telegina; Andrey A. Buglak; Elena V. Lukinova; Eduard I. Madirov; Yuliya L. Vechtomova; Mikhail S. Kritsky; Vadim V. Semashko

doi:10.1117/12.2555041

11 December 2019 The role of photochemical transformations of tetrahydrobiopterin in the pathogenesis and phototherapy of vitiligo

Alexey S. Nizamutdinov, Taisia A. Telegina, Andrey A. Buglak, Elena V. Lukinova, Eduard I. Madirov, Yuliya L. Vechtomova, Mikhail S. Kritsky, Vadim V. Semashko

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Proceedings Volume 11322, XIV International Conference on Pulsed Lasers and Laser Applications; 1132207 (2019) https://doi.org/10.1117/12.2555041
Event: XIV International Conference on Pulsed Lasers and Laser Applications (AMPL-2019), 2019, Tomsk, Russian Federation

Abstract

Melanogenesis disorder leads to several pathologies, including vitiligo. Tetrahydrobiopterin (H₄Bip) as the phenylalanine 4-hydroxylase coenzyme catalyzes the oxidation of phenylalanine to tyrosine (a melanin precursor). H4Bip is easily oxidized by oxygen in vivo and in vitro. Vitiligo is accompanied by three-fivefold increased de-novo synthesis of H₄Bip, its excess and its further oxidation are essential factors in the pathogenesis of vitiligo. We have demonstrated that pterin products of H₄Bip autoxidation (dihydropterin (H₂Ptr), dihydroxanthopterin and pterin) predominate over biopterin products (dihydrobiopterin (H₂Bip) and biopterin). It was shown that ultraviolet (UV) irradiation accelerates the autoxidation while the products of oxidative degradation of H₄Bip act as photosensitizers. Photosensitized oxidation of H₄Bip can contribute to the pathogenesis of vitiligo. The main distinguishing feature of UV photooxidation of H₄Bip from autoxidation was the formation of dihydropterin (Н₂Ptr)₂ and dihydrobiopterin (Н₂Bip)₂ dimers. Here we reported on the dependences of the photodimerization reaction on the wavelength and intensity of radiation using xenon lamps and UV tunable lasers as sources of UV radiation. It was shown that UV irradiation with a laser is more efficient than that with xenon lamp. It was established that the greatest number of dimers were formed by irradiating the H₄Bip solution by radiation with a wavelength in the range 308-312 nm. The data obtained are discussed in the context of UVB narrowband vitiligo phototherapy.

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Alexey S. Nizamutdinov, Taisia A. Telegina, Andrey A. Buglak, Elena V. Lukinova, Eduard I. Madirov, Yuliya L. Vechtomova, Mikhail S. Kritsky, and Vadim V. Semashko "The role of photochemical transformations of tetrahydrobiopterin in the pathogenesis and phototherapy of vitiligo", Proc. SPIE 11322, XIV International Conference on Pulsed Lasers and Laser Applications, 1132207 (11 December 2019); https://doi.org/10.1117/12.2555041

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KEYWORDS

Absorption

Ultraviolet radiation

Oxidation

Lamps

Oxygen

Phototherapy

Pulsed laser operation

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