Waveguide chip coupled with microfluidics enables super-resolution live-cell imaging

Matteo Boninsegna; Nikhil Jayakumar; Firehun Tsige Dullo; Peter A. G. McCourt; Thomas Huser; Balpreet Singh Ahluwalia; Antoni Homs Corbera

doi:10.1117/12.2568385

21 August 2020 Waveguide chip coupled with microfluidics enables super-resolution live-cell imaging

Matteo Boninsegna, Nikhil Jayakumar, Firehun Tsige Dullo, Peter A. G. McCourt, Thomas Huser, Balpreet Singh Ahluwalia, Antoni Homs Corbera

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Proceedings Volume 11483, Novel Optical Systems, Methods, and Applications XXIII; 114830T (2020) https://doi.org/10.1117/12.2568385
Event: SPIE Optical Engineering + Applications, 2020, Online Only

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Abstract

Chip-based optical nanoscopy, relying on single molecule localization microscopy has recently been demonstrated to reach 70 nm lateral resolution over wide fields of view (500 µm x 500 µm). To make this technique more sustainable for live-cell imaging we embedded a photonic chip into a microfluidic support that is able to perfuse and thermalize the samples. In this way specimens are maintained under physiological conditions during the imaging which can be a timeconsuming process. The system consists of a multilayer chip with the size of a glass coverslip (60 mm × 24 mm). The sample is illuminated using waveguides that are fabricated from high refractive index material. The waveguide hosts a chamber (17.3 μl) where cells are seeded and perfused with medium. A thin layer (188 µm) of cyclic olefin polymer (COP) seals the chamber and allows optical image acquisition. A thermalizing solution is perfused from the bottom to accurately warm up/cool down the waveguide in a range of 5°C - 45°C. Thus, samples are kept at the proper temperature. As proof of concept and verification of super-resolution imaging, we imaged fluorescent beads perfused across the coated surface (fibronectin 0.2 mg/ml) of the chip, which is needed to guarantee proper cell-to-substrate adhesion.

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Matteo Boninsegna, Nikhil Jayakumar, Firehun Tsige Dullo, Peter A. G. McCourt, Thomas Huser, Balpreet Singh Ahluwalia, and Antoni Homs Corbera "Waveguide chip coupled with microfluidics enables super-resolution live-cell imaging", Proc. SPIE 11483, Novel Optical Systems, Methods, and Applications XXIII, 114830T (21 August 2020); https://doi.org/10.1117/12.2568385

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