High-speed lens-free holographic microscope for biomolecular sensing

Zhen Xiong; Euan McLeod

doi:10.1117/12.2568691

21 August 2020 High-speed lens-free holographic microscope for biomolecular sensing

Zhen Xiong, Euan McLeod

Proceedings Volume 11509, Optics and Photonics for Information Processing XIV; 115090D (2020) https://doi.org/10.1117/12.2568691
Event: SPIE Optical Engineering + Applications, 2020, Online Only

Abstract

We implemented a lens-free holographic microscope (LFHM) that can image ~105 2-μm microspheres in solution over an ultra-large field-of-view >15 mm2 with sub-micron resolution and enhanced signal-to-noise ratio in less than 1 second. This performance is achieved using a high-speed hardware design for multi-frame pixel super-resolution and a novel sparsity-promoting reconstruction algorithm. We use the microscope as a biomolecular sensor to detect and quantify NeutrAvidin protein molecules. We coat 2-μm microspheres with biotin, which binds strongly to NAv, causing observable bead binding and clustering. This is quantified through an automated image processing algorithm and is used to infer NAv concentrations.

Conference Presentation

Citation Download Citation

Zhen Xiong and Euan McLeod "High-speed lens-free holographic microscope for biomolecular sensing", Proc. SPIE 11509, Optics and Photonics for Information Processing XIV, 115090D (21 August 2020); https://doi.org/10.1117/12.2568691

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