Assessing membrane charging by alternating electric fields in giant unilamellar vesicles

Allen Kiester; Stacey L. Martens; Zachary Coker; Mara Casebeer; Joel Bixler; Esin B. Sözer; Bennett L. Ibey

doi:10.1117/12.2578299

5 March 2021 Assessing membrane charging by alternating electric fields in giant unilamellar vesicles

Allen Kiester, Stacey L. Martens, Zachary Coker, Mara Casebeer, Joel Bixler, Esin B. Sözer, Bennett L. Ibey

Author Affiliations +

Proceedings Volume 11640, Optical Interactions with Tissue and Cells XXXII; 1164009 (2021) https://doi.org/10.1117/12.2578299
Event: SPIE BiOS, 2021, Online Only

Abstract

Our group recently published direct observation of membrane charging in FluoVolt labeled CHO-K1 cells by nanosecond electrical pulses using a streak camera. Using this technique, called Streak Camera Microscopy (SCM), we imaged the membrane charging dynamics in giant unilamellar vesicles (GUVs) during AC exposures up to 6 MHz and compared these results to existing capacitive circuit models of membranes. This work shows further application of Streak Camera Microscopy for evaluation of high speed biological events.

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Citation Download Citation

Allen Kiester, Stacey L. Martens, Zachary Coker, Mara Casebeer, Joel Bixler, Esin B. Sözer, and Bennett L. Ibey "Assessing membrane charging by alternating electric fields in giant unilamellar vesicles", Proc. SPIE 11640, Optical Interactions with Tissue and Cells XXXII, 1164009 (5 March 2021); https://doi.org/10.1117/12.2578299

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