Quantitative phase-contrast by using a modified phase retrieval algorithm

Nathaniel Hai; Joseph Rosen

doi:10.1117/12.2576495

5 March 2021 Quantitative phase-contrast by using a modified phase retrieval algorithm

Nathaniel Hai, Joseph Rosen

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116530S (2021) https://doi.org/10.1117/12.2576495
Event: SPIE BiOS, 2021, Online Only

Abstract

Spatial-filtering-based quantitative phase imaging of transparent objects is demonstrated. By combining a standard phase contrast microscopy technique with an optical-system-mimicking Gerchberg-Saxton phase retrieval algorithm, we successfully transformed a qualitative phase map into an accurate quantitative phase distribution of the examined object. Feeding a phase-induced intensity image into the modified phase retrieval algorithm is found to facilitate the convergence and ambiguities issues associated with the standard algorithms. Accurate phase recovery for two different kinds of objects supports the use of the suggested method as a single-shot quantitative phase contrast microscope.

Conference Presentation

Citation Download Citation

Nathaniel Hai and Joseph Rosen "Quantitative phase-contrast by using a modified phase retrieval algorithm", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116530S (5 March 2021); https://doi.org/10.1117/12.2576495

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