Stain-free virtual histology of Bombus terrestris compound eyes by laboratory phase-contrast nano-CT

Jenny Romell; Vun Wen Jie; Arttu Miettinen; Emily Baird; Hans M. Hertz

doi:10.1117/12.2595737

1 August 2021 Stain-free virtual histology of Bombus terrestris compound eyes by laboratory phase-contrast nano-CT

Jenny Romell, Vun Wen Jie, Arttu Miettinen, Emily Baird, Hans M. Hertz

Proceedings Volume 11840, Developments in X-Ray Tomography XIII; 118400O (2021) https://doi.org/10.1117/12.2595737
Event: SPIE Optical Engineering + Applications, 2021, San Diego, California, United States

Abstract

The visual systems of pollinating insects are studied to understand the effects of a changing environment, and are to date imaged using microscopy or micro-CT. Microscopy only allows two-dimensional imaging and conventional micro-CT requires heavy-metal staining of the samples. Here we present virtual histology of compound eyes of bumblebee (Bombus terrestris) using a laboratory nano-CT system. Propagation-based phase-contrast CT allows three-dimensional imaging of samples without using any staining. Micrometre-resolution images of the microanatomy of the eyes were reconstructed, and the features identified in CT (cornea, crystalline cones, pigment, photoreceptor cells, basement membrane) were confirmed with electron microscopy.

Conference Presentation

Citation Download Citation

Jenny Romell, Vun Wen Jie, Arttu Miettinen, Emily Baird, and Hans M. Hertz "Stain-free virtual histology of Bombus terrestris compound eyes by laboratory phase-contrast nano-CT", Proc. SPIE 11840, Developments in X-Ray Tomography XIII, 118400O (1 August 2021); https://doi.org/10.1117/12.2595737

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