Fluorescence lifetime phantoms based on quenched coumarin derivatives

Christian Freymüller; Sviatlana Kalinina; Angelika Rück; Ronald Sroka; Adrian Rühm

doi:10.1117/12.2615823

7 December 2021 Fluorescence lifetime phantoms based on quenched coumarin derivatives

Christian Freymüller, Sviatlana Kalinina, Angelika Rück, Ronald Sroka, Adrian Rühm

Author Affiliations +

Proceedings Volume 11922, Advances in Microscopic Imaging III; 119221K (2021) https://doi.org/10.1117/12.2615823
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

Liquid reference samples for 2-photon fluorescence lifetime investigations were developed, based on coumarin 1 and 6 and 4-hydroxy-TEMPO as quencher. Spectral and fluorescence lifetime characteristics similar to intracellular NADH and FAD were achieved.

Citation Download Citation

Christian Freymüller, Sviatlana Kalinina, Angelika Rück, Ronald Sroka, and Adrian Rühm "Fluorescence lifetime phantoms based on quenched coumarin derivatives", Proc. SPIE 11922, Advances in Microscopic Imaging III, 119221K (7 December 2021); https://doi.org/10.1117/12.2615823

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