Enhanced Raman techniques for infection diagnostics

Niall Hanrahan; Adam Lister; Callum Highmore; Leena Rajith; Ekaterina Avershina; Jawad Ali; Rafi Ahmad; Jeremy Webb; Sumeet Mahajan

doi:10.1117/12.2635324

3 October 2022 Enhanced Raman techniques for infection diagnostics

Niall Hanrahan, Adam Lister, Callum Highmore, Leena Rajith, Ekaterina Avershina, Jawad Ali, Rafi Ahmad, Jeremy Webb, Sumeet Mahajan

Author Affiliations +

Proceedings Volume 12203, Enhanced Spectroscopies and Nanoimaging 2022; 1220303 (2022) https://doi.org/10.1117/12.2635324
Event: SPIE Nanoscience + Engineering, 2022, San Diego, California, United States

Abstract

In this paper we describe our recent work in multi-excitation surface enhanced Raman spectroscopy (MX-SERS), and its application for robust strain-level bacteria identification. The development of MX-SERS follows directly from our previous work in rapid bacterial identification using multi-excitation Raman spectroscopy (MX-Raman), which enabled highly accurate (up to 99.75%) strain-level distinction of bacteria, including antibiotic resistant strains of bacteria and from within complex media. In this work we use the strong wavelength dependence of both the Raman scattering cross-section and the surface plasmon to demonstrate a novel capability in bacteria identification. Compared to MX-Raman, MX-SERS has up to 8x faster data acquisition speed as well as up to 4000x lower laser power incident on the sample. Furthermore, we fabricate SERS-active substrates with a simple and low-cost fabrication method that can be adapted to fit a chosen wavelength regime. This combination of strain-level sensitivity and high-speed detection, combined with a low-cost SERS substrate, has strong potential applications in clinical diagnostics, and could be integrated within a real-world pathogen detection workflow.

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Citation Download Citation

Niall Hanrahan, Adam Lister, Callum Highmore, Leena Rajith, Ekaterina Avershina, Jawad Ali, Rafi Ahmad, Jeremy Webb, and Sumeet Mahajan "Enhanced Raman techniques for infection diagnostics", Proc. SPIE 12203, Enhanced Spectroscopies and Nanoimaging 2022, 1220303 (3 October 2022); https://doi.org/10.1117/12.2635324

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