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29 April 2022 Spectrophotometric determination of cysteine hydrochloride by phosphomolybdic blue
Xinrong Wen, Changqing Tu
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Proceedings Volume 12255, 2022 International Conference on Optoelectronic Information and Functional Materials (OIFM 2022); 1225528 (2022) https://doi.org/10.1117/12.2640357
Event: 2022 International Conference on Optoelectronic Information and Functional Materials (OIFM 2022), 2022, Chongqing, China
Abstract
In H2SO4 medium, phosphorus molybdenum heteropoly acid formed from H2PO4 - reacts with Mo7O246- can be reduced by hydrosulfuryl(-SH) in cysteine hydrochloride to from phosphomolybdic blue which its maximum absorption wavelength is 717.2 nm. Beer's law is obeyed between the mass concentration of cysteine hydrochloride and the absorbance of phosphomolybdic blue, and the content of cysteine hydrochloride can be determined based on the measuring of the absorbance of phosphomolybdic blue. A novel method for the spectrophotometric determination of cysteine hydrochloride by phosphomolybdic blue has been established. A good linear relationship is obtained between absorbance and the concentration of cysteine hydrochloride in the range of 0.008160～0.1469 mg/mL, the equation of the linear regression is A=0.0305+9.9918C(mg/mL), and the linear correlation coefficient is 0.9995. This proposed method had been successfully applied to determinate cysteine hydrochloride, and the result agreed well with those by pharmacopoeial method.
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Xinrong Wen and Changqing Tu "Spectrophotometric determination of cysteine hydrochloride by phosphomolybdic blue", Proc. SPIE 12255, 2022 International Conference on Optoelectronic Information and Functional Materials (OIFM 2022), 1225528 (29 April 2022); https://doi.org/10.1117/12.2640357
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KEYWORDS
Absorbance
Molybdenum
Phosphorus
Absorption
Spectrophotometry
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