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15 March 2023 Multi-spectral imaging in mammalian cells using a home-built LSFM system
Aiswarya K. S., Rinsa S. R., Rahul Sharma, Srinivasa Murty Srinivasula, Mayanglambam Suheshkumar Singh
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Proceedings Volume 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; 123830L (2023) https://doi.org/10.1117/12.2649890
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Light sheet fluorescence microscopy (LSFM) works on fluorescence imaging, employing target-specific fluorescent proteins to label regions of interest in a sample. In this way, analyzing and manipulating information from different targets in a specimen requires labeling the specimen using multiple fluorophores. Multispectral imaging helps identify several target molecules simultaneously at once, thereby retrieving information from the entire volume of the sample. Here we report multispectral imaging studies on mammalian cells using our in-house built multispectral LSFM imaging system. Experimental studies were conducted on mammalian cells in alive conditions, and results are provided.
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Aiswarya K. S., Rinsa S. R., Rahul Sharma, Srinivasa Murty Srinivasula, and Mayanglambam Suheshkumar Singh "Multi-spectral imaging in mammalian cells using a home-built LSFM system", Proc. SPIE 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, 123830L (15 March 2023); https://doi.org/10.1117/12.2649890
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KEYWORDS
Multispectral imaging
Imaging systems
Image fusion
Fluorescence imaging
Biological imaging
Fluorophores
Laser sources
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