EDoF-Miniscope: a computational miniscope for extended depth-of-field neural imaging

Joseph Greene; Yujia Xue; Jeffrey Alido; Alex Matlock; Guorong Hu; Kivilcim Kiliç; Ian Davison; Lei Tian

doi:10.1117/12.2648565

16 March 2023 EDoF-Miniscope: a computational miniscope for extended depth-of-field neural imaging

Joseph Greene, Yujia Xue, Jeffrey Alido, Alex Matlock, Guorong Hu, Kivilcim Kiliç, Ian Davison, Lei Tian

Author Affiliations +

Proceedings Volume 12388, Adaptive Optics and Wavefront Control for Biological Systems IX; 1238808 (2023) https://doi.org/10.1117/12.2648565
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We demonstrate an extended-depth-of-field miniscope (EDoF-Miniscope) which utilizes an optimized binary diffractive optical element (DOE) to achieve a 2.8x axial elongation in twin foci when integrated on the pupil plane. We optimize our DOE through a genetic algorithm, which utilizes a Fourier optics forward model to consider the native aberrations of the primary gradient refractive index (GRIN) lens, optical property of the submersion media, the geometric effects of the target fluorescent sources and axial intensity loss from tissue scattering to create a robust EDoF. We demonstrate that our platform achieves high contrast signals that can be recovered through a simple filter across 5-μm and 10-μm beads embedded in scattering phantoms, and fixed mouse brain samples.

Conference Presentation

Citation Download Citation

Joseph Greene, Yujia Xue, Jeffrey Alido, Alex Matlock, Guorong Hu, Kivilcim Kiliç, Ian Davison, and Lei Tian "EDoF-Miniscope: a computational miniscope for extended depth-of-field neural imaging", Proc. SPIE 12388, Adaptive Optics and Wavefront Control for Biological Systems IX, 1238808 (16 March 2023); https://doi.org/10.1117/12.2648565

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