Disinfection of Escherichia coli and Salmonella enterica using the contamination sanitization inspection and disinfection (CSI-D) device

J. McCoy Sanders; V. Alarcon; G. Marquis; A. Tabb; J. Van Kessel; J. Sonnier; B. Haley; I. Baek; J. Qin; M. Kim; F. Vasefi; S. Sokolov; R. Hellberg

doi:10.1117/12.2664049

13 June 2023 Disinfection of Escherichia coli and Salmonella enterica using the contamination sanitization inspection and disinfection (CSI-D) device

J. McCoy Sanders, V. Alarcon, G. Marquis, A. Tabb, J. Van Kessel, J. Sonnier, B. Haley, I. Baek, J. Qin, M. Kim, F. Vasefi, S. Sokolov, R. Hellberg

Author Affiliations +

Proceedings Volume 12545, Sensing for Agriculture and Food Quality and Safety XV; 125450F (2023) https://doi.org/10.1117/12.2664049
Event: SPIE Defense + Commercial Sensing, 2023, Orlando, Florida, United States

Conference Poster

Abstract

Escherichia coli and Salmonella enterica are significant causes of gastrointestinal disease globally. The Contamination Sanitization Inspection and Disinfection (CSI-D) device is a handheld fluorescence-based imaging system that disinfects food contact surfaces using ultraviolet-C (UVC) illumination. The goal of this study was to determine the optimal parameters for disinfection of E. coli and S. enterica using the CSI-D system. E. coli and S. enterica Enteritidis, Newport, Typhimurium, and Javiana were grown on selective media, followed by transfer to Luria Bertani broth. After overnight incubation, the cultures were diluted and spread-plated on L-agar. The plates were exposed to high-intensity (10 mW/cm2) or low-intensity (5 mW/cm2) UVC for 1 s, 3 s, or 5 s. Exposed and control plates were incubated at room temperature for 2-3 h, then overnight at 37°C. The resulting colonies were counted and compared to control plates. Three trials were conducted on separate days. The average of the trials showed that exposure times of 3-5 s at either intensity resulted in effective and consistent destruction of E. coli and S. enterica. The minimum reduction at 3 s exposure for both intensities was 96%, with a maximum of 100%. The 1 s exposure time showed inconsistent results, with a 0-61.5% survival rate. The results of this study show that exposure to UVC for at least 3 s is required to achieve consistent disinfection of 96-100% for generic E. coli and S. enterica.

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J. McCoy Sanders, V. Alarcon, G. Marquis, A. Tabb, J. Van Kessel, J. Sonnier, B. Haley, I. Baek, J. Qin, M. Kim, F. Vasefi, S. Sokolov, and R. Hellberg "Disinfection of Escherichia coli and Salmonella enterica using the contamination sanitization inspection and disinfection (CSI-D) device", Proc. SPIE 12545, Sensing for Agriculture and Food Quality and Safety XV, 125450F (13 June 2023); https://doi.org/10.1117/12.2664049

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