Decoupling of  complex refractive index in transport of intensity diffraction tomography microscopy

Zhidong Bai; Qian Chen; Jiaji Li; Chao Zuo

doi:10.1117/12.2667065

27 January 2023 Decoupling of complex refractive index in transport of intensity diffraction tomography microscopy

Zhidong Bai, Qian Chen, Jiaji Li, Chao Zuo

Author Affiliations +

Proceedings Volume 12550, International Conference on Optical and Photonic Engineering (icOPEN 2022); 1255029 (2023) https://doi.org/10.1117/12.2667065
Event: International Conference on Optical and Photonic Engineering (icOPEN 2022), 2022, ONLINE, China

Abstract

In this paper, we demonstrate the decoupling of complex refractive index in transport of intensity diffraction tomography (TIDT) microscopy under partially coherent illumination. Specifically, with a closed-form Tikhonov-regularized solution, this method enables the decoupling of the RI and absorption from the two through-focus intensity stacks. We compared the reconstruction performance of our proposed method with other optical diffraction tomography methods by means of numerical simulations. The results showed that our method provides RI and absorption images of 3D resolution targets with minimal artifacts. Finally, we apply the technique to imaging biological samples, e.g., the Thalassiosira diatoms, revealing the intrinsic structural diversity of the biological samples.

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Zhidong Bai, Qian Chen, Jiaji Li, and Chao Zuo "Decoupling of complex refractive index in transport of intensity diffraction tomography microscopy", Proc. SPIE 12550, International Conference on Optical and Photonic Engineering (icOPEN 2022), 1255029 (27 January 2023); https://doi.org/10.1117/12.2667065

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