Identification of individual red blood cells by single-cell Raman spectroscopy combined with machine learning

Yiming Liu; Zhehai Zhou

doi:10.1117/12.2646675

20 January 2023 Identification of individual red blood cells by single-cell Raman spectroscopy combined with machine learning

Yiming Liu, Zhehai Zhou

Author Affiliations +

Proceedings Volume 12560, AOPC 2022: Biomedical Optics; 1256005 (2023) https://doi.org/10.1117/12.2646675
Event: Applied Optics and Photonics China 2022 (AOPC2022), 2022, Beijing, China

Abstract

Raman spectroscopy, a “fingerprint” spectrum of substances, can be used to characterize various biological and chemical samples. To allow for blood classification using single-cell Raman spectroscopy, several machine learning algorithms were implemented and compared. A single-cell laser optical tweezer Raman spectroscopy system was established to obtain the Raman spectra of red blood cells. The Boruta algorithm extracted the spectral feature frequency shift, reduced the spectral dimension, and determined the essential features that affect classification. Next, seven machine learning classification models and deep learning model without dimensionality reduction are analyzed and compared based on the classification accuracy, precision, and recall indicators. The results show that support vector machines and convolutional neural network are the two most appropriate machine learning algorithms for single-cell Raman spectrum blood classification, and the findings provide essential guidance for future research studies.

Citation Download Citation

Yiming Liu and Zhehai Zhou "Identification of individual red blood cells by single-cell Raman spectroscopy combined with machine learning", Proc. SPIE 12560, AOPC 2022: Biomedical Optics, 1256005 (20 January 2023); https://doi.org/10.1117/12.2646675

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available