Contrary microscope: opportunities and advantages: optical design

Dmitry N. Frolov; Olga A. Vinogradova; Alexey D. Frolov; Alexandr D. Pavlii

doi:10.1117/12.2678324

2 October 2023 Contrary microscope: opportunities and advantages: optical design

Dmitry N. Frolov, Olga A. Vinogradova, Alexey D. Frolov, Alexandr D. Pavlii

Proceedings Volume 12665, Novel Optical Systems, Methods, and Applications XXVI; 126650W (2023) https://doi.org/10.1117/12.2678324
Event: SPIE Optical Engineering + Applications, 2023, San Diego, California, United States

Conference Poster

Abstract

In the classical layout of the light microscope, when the objective is used as a projection system, and eyepiece as an observation, the redistribution of overall and functional parameters is proposed. In the classical layout typically, own linear magnification for objectives is from 2.5 to 100x, and eyepieces from 5 to 30x. It is proposed to use objectives of magnification of not more than 10-15x but having extremely achievable input numerical apertures. In this case, the range of increases of the eyepieces expands to 50x and even 100x. We offer examples of optical designs of objectives and eyepieces. The objective 10x magnification has NA=0.90 and 12.5x NA=1.20 of the water immersion. The linear field in the space of images is 20 mm. In addition, examples of optical designs of eyepieces 50x and 100x are offered too.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Dmitry N. Frolov, Olga A. Vinogradova, Alexey D. Frolov, and Alexandr D. Pavlii "Contrary microscope: opportunities and advantages: optical design", Proc. SPIE 12665, Novel Optical Systems, Methods, and Applications XXVI, 126650W (2 October 2023); https://doi.org/10.1117/12.2678324

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available