Using 3D printed optical elements for multifocal image scanning microscopy

Jay Christopher; Mark Donnachie; Liam Rooney; Gail McConnell; Deepak Uttamchandani; Ralf Bauer

doi:10.1117/12.3002507

12 March 2024 Using 3D printed optical elements for multifocal image scanning microscopy

Jay Christopher, Mark Donnachie, Liam Rooney, Gail McConnell, Deepak Uttamchandani, Ralf Bauer

Proceedings Volume 12827, Multiscale Imaging and Spectroscopy V; 1282702 (2024) https://doi.org/10.1117/12.3002507
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We present a small footprint and affordable implementation of a multifocal image scanning microscope (ISM), utilizing both a microelectromechanical system (MEMS) micromirror for flexible optical excitation control, and multifocal patterned illumination using a custom 3D printed optical quality lenslet array. We highlight the individual element performance and demonstrate its use in fluorescence imaging to allow comparison of the affordable and customizable approach with its commercial counterpart.

Citation Download Citation

Jay Christopher, Mark Donnachie, Liam Rooney, Gail McConnell, Deepak Uttamchandani, and Ralf Bauer "Using 3D printed optical elements for multifocal image scanning microscopy", Proc. SPIE 12827, Multiscale Imaging and Spectroscopy V, 1282702 (12 March 2024); https://doi.org/10.1117/12.3002507

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