Evaluation of the limit of detection of the localized surface plasmon resonance label-free biosensor

M. P. Mcoyi; M. Lugongolo; C. Williamson; K. Mpofu; S. Ombinda-Lemboumba; S. Ndlovu; P. Mthunzi-Kufa

doi:10.1117/12.3002239

12 March 2024 Evaluation of the limit of detection of the localized surface plasmon resonance label-free biosensor

M. P. Mcoyi, M. Lugongolo, C. Williamson, K. Mpofu, S. Ombinda-Lemboumba, S. Ndlovu, P. Mthunzi-Kufa

Proceedings Volume 12840, Optical Interactions with Tissue and Cells XXXV; 128400G (2024) https://doi.org/10.1117/12.3002239
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Plasmonic biosensing is an area of research where scientists are developing novel devices and concepts. The validation of these devices requires thorough testing of key characteristics, including accuracy, specificity, robustness, and linearity, all of which are interconnected with a system's limit of detection. By optimizing parameters to meet these criteria, the development of highly sensitive and specific biosensing devices can be facilitated, allowing for effective performance comparisons with other biosensing devices. The limit of detection is a measure of the ability to detect small traces of molecules in each solution, and it can be used to measure the performance of a biosensing system. In this study, we evaluated the limit of detection of a home-built localized surface plasmon resonance (LSPR) system. To this end, gold-coated and titanium-coated slides were exposed to neutravidin and biotinylated probes at varying concentrations ranging from 100 μM to 6.125 μM, without the presence of an analyte. By analyzing the transmitted intensity against concentration, we constructed a reliable sensing curve. Through linear regression analysis, we determined the slope of the linear calibration function with high precision. By evaluating the limit of detection, this study will contribute to the understanding of the performance capabilities of the localized surface plasmon resonance technique. The findings presented here serve as a valuable benchmark for developing and comparing highly sensitive and specific biosensing devices.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

M. P. Mcoyi, M. Lugongolo, C. Williamson, K. Mpofu, S. Ombinda-Lemboumba, S. Ndlovu, and P. Mthunzi-Kufa "Evaluation of the limit of detection of the localized surface plasmon resonance label-free biosensor", Proc. SPIE 12840, Optical Interactions with Tissue and Cells XXXV, 128400G (12 March 2024); https://doi.org/10.1117/12.3002239

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