Quantitative photoacoustic microscopy for methylene blue concentration mapping in agar phantoms at multiple wavelengths

Deepayan Samanta; Piyao Narumbam; Arijit Paramanick; M. Suheshkumar Singh

doi:10.1117/12.3003665

12 March 2024 Quantitative photoacoustic microscopy for methylene blue concentration mapping in agar phantoms at multiple wavelengths

Deepayan Samanta, Piyao Narumbam, Arijit Paramanick, M. Suheshkumar Singh

Author Affiliations +

Proceedings Volume 12842, Photons Plus Ultrasound: Imaging and Sensing 2024; 128421F (2024) https://doi.org/10.1117/12.3003665
Event: SPIE BiOS, 2024, San Francisco, California, United States

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Abstract

This work explores the use of quantitative photoacoustic microscopy to map the concentrations of methylene blue in agar phantoms. Our investigation covers wavelengths from 700 nm to 750 nm and looks at concentrations of 5 mM, 10 mM, and 15 mM. Through a detailed investigation of the photoacoustic response, the multi-wavelength method provides information about molecule distributions. Our results highlight this methodology’s potential for accurate concentration mapping, with prospective applications in the clinical and biological areas. By giving a systematic investigation of methylene blue concentrations, this work adds to the growing area of photoacoustic microscopy and highlights the value and adaptability of multi-wavelength imaging for molecular mapping. The findings could lead to improved molecular imaging and have consequences for researchers and practitioners in the fields of biomedical optics, spectroscopy, and photoacoustic imaging.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Deepayan Samanta, Piyao Narumbam, Arijit Paramanick, and M. Suheshkumar Singh "Quantitative photoacoustic microscopy for methylene blue concentration mapping in agar phantoms at multiple wavelengths", Proc. SPIE 12842, Photons Plus Ultrasound: Imaging and Sensing 2024, 128421F (12 March 2024); https://doi.org/10.1117/12.3003665

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