Simultaneous acousto-optic modulation and multiphoton fluorescence imaging provides functional imaging and new image details

Ezra Guralnik; Behnam Tayebi; Yi Yuan; Justin Little; Michal Balberg; Shy Shoham

doi:10.1117/12.3003387

12 March 2024 Simultaneous acousto-optic modulation and multiphoton fluorescence imaging provides functional imaging and new image details

Ezra Guralnik, Behnam Tayebi, Yi Yuan, Justin Little, Michal Balberg, Shy Shoham

Author Affiliations +

Proceedings Volume 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; 128470F (2024) https://doi.org/10.1117/12.3003387
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

An advantage of two-photon microscopy is its sectioning capabilities, however, in practice it can often suffer from out of focus contamination. Here, we report the new application of strong acousto-optic modulation of two-photon excited fluorescence. We demonstrate how this effect enhances image detail and contrast and improves optical sectioning in vivo. Requiring no changes to the optical path or image acquisition parameters, our method is simple to implement and compatible with standard multiphoton microscopes.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Ezra Guralnik, Behnam Tayebi, Yi Yuan, Justin Little, Michal Balberg, and Shy Shoham "Simultaneous acousto-optic modulation and multiphoton fluorescence imaging provides functional imaging and new image details", Proc. SPIE 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, 128470F (12 March 2024); https://doi.org/10.1117/12.3003387

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