3-D printed compact and cost-effective lens-less digital inline holographic microscopy setup for bio-imaging applications

Deep Arya; Utkarsh Rawat; Rishikesh Kulkarni; Pranab K. Mondal; Prithwijit Guha

doi:10.1117/12.3002384

13 March 2024 3-D printed compact and cost-effective lens-less digital inline holographic microscopy setup for bio-imaging applications

Deep Arya, Utkarsh Rawat, Rishikesh Kulkarni, Pranab K. Mondal, Prithwijit Guha

Author Affiliations +

Proceedings Volume 12910, Practical Holography XXXVIII: Displays, Materials, and Applications; 129100Q (2024) https://doi.org/10.1117/12.3002384
Event: SPIE OPTO, 2024, San Francisco, California, United States

Conference Poster

Abstract

The applicability of the proposed 3-D printed lens-less digital inline holographic microscopy setup is demonstrated with bio-imaging examples. The direct object illumination with an elliptical beam from laser diode avoids the spatial filtering and beam collimation assembly which significantly reduces the setup complexity and cost. The use of Raspberry Pi camera sensor for hologram recording further reduces the cost without compromising on the hologram reconstructed image quality. The adjustable magnification in the range of 1.1X-1.8X is achieved. We believe that the proposed DIHM setup can be an important imaging tool especially in the resource constrained applications.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Deep Arya, Utkarsh Rawat, Rishikesh Kulkarni, Pranab K. Mondal, and Prithwijit Guha "3-D printed compact and cost-effective lens-less digital inline holographic microscopy setup for bio-imaging applications", Proc. SPIE 12910, Practical Holography XXXVIII: Displays, Materials, and Applications, 129100Q (13 March 2024); https://doi.org/10.1117/12.3002384

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