Spectral interferometric polarization imaging using broadband CARS

Ryan Muddiman; Syed Baryalay; Matthew McCartney; Grace A. Hoysted; Bryan Hennelly

doi:10.1117/12.3017645

18 June 2024 Spectral interferometric polarization imaging using broadband CARS

Ryan Muddiman, Syed Baryalay, Matthew McCartney, Grace A. Hoysted, Bryan Hennelly

Proceedings Volume 12996, Unconventional Optical Imaging IV; 129960P (2024) https://doi.org/10.1117/12.3017645
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Broadband CARS is commonly used to study tissue sections of biological samples utilizing the inherent vibrational contrast present due to molecular vibrations. This technique however, is notably hindered by the ubiquitous non-resonant background (NRB) that plagues the interpretation of images. Nevertheless, a promising avenue is polarization suppression which was previously reported for single frequency CARS and has shown efficacy in NRB removal. Here, we employ polarization suppression using two acquisitions for interferometric NRB rejection, which has previously been applied to polymers and liquids. The spectral interferometric method requires only passive polarization optics, in addition to a BCARS system. The method was applied to the BCARS imaging of fungal spores and compared to the spectra obtained from the Kramers-Kronig phase retrieval algorithm.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Ryan Muddiman, Syed Baryalay, Matthew McCartney, Grace A. Hoysted, and Bryan Hennelly "Spectral interferometric polarization imaging using broadband CARS", Proc. SPIE 12996, Unconventional Optical Imaging IV, 129960P (18 June 2024); https://doi.org/10.1117/12.3017645

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