Design of a portable fluorescence imaging platform for on-site detection target analyte by loop-medicated isothermal amplification

Noah Boursier; Junwoo Jang; Awadhoot M. Ghatge; Kevin Lim; Thomas R. McClure; J. Paul Robinson; Euiwon Bae

doi:10.1117/12.3016161

6 June 2024 Design of a portable fluorescence imaging platform for on-site detection target analyte by loop-medicated isothermal amplification

Noah Boursier, Junwoo Jang, Awadhoot M. Ghatge, Kevin Lim, Thomas R. McClure, J. Paul Robinson, Euiwon Bae

Author Affiliations +

Proceedings Volume 13060, Sensing for Agriculture and Food Quality and Safety XVI; 130600A (2024) https://doi.org/10.1117/12.3016161
Event: SPIE Defense + Commercial Sensing, 2024, National Harbor, Maryland, United States

Abstract

With the development and expansion of the internet of things, many scientific and engineering instruments are leaving the benchtop restriction and moving on to provide on-site detection. On-site detection requires a complete miniaturization of a benchtop system while maintaining a similar performance with respect to the analyte detection sensitivity. In addition, due to the mobile nature, utilizing a battery source is required. Here we present a portable loop-medicated isothermal amplification detection system for on-site detection and amplification of target analyte via fluorescence detection. The digital twin design incorporates three major components: an isothermal heating chamber, light-tight enclosure for sample insert, and fluorescence imaging system via micro-controllers. The isothermal heating chamber was designed with Peltier heater to provide small form factor accurate temperature control. For light-tight enclosure is a 3D printed device that allows DNA samples to be inserted and fluorescent images to be taken within the chamber. Lastly, fluorescent imaging system operates with a stand-alone camera connected to an Arduino micro-controller. Excitation is provided by blue colored LED and emission is detected via long-pass filter that matches the emission spectrum.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Noah Boursier, Junwoo Jang, Awadhoot M. Ghatge, Kevin Lim, Thomas R. McClure, J. Paul Robinson, and Euiwon Bae "Design of a portable fluorescence imaging platform for on-site detection target analyte by loop-medicated isothermal amplification", Proc. SPIE 13060, Sensing for Agriculture and Food Quality and Safety XVI, 130600A (6 June 2024); https://doi.org/10.1117/12.3016161

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