Real-time structured illumination microscopy based on Fourier ptychographic

Jixiang Zhou; Jiaming Qian; Yuzhen Zhang

doi:10.1117/12.3044481

26 September 2024 Real-time structured illumination microscopy based on Fourier ptychographic

Jixiang Zhou, Jiaming Qian, Yuzhen Zhang

Proceedings Volume 13282, Second Advanced Imaging and Information Processing Conference (AIIP 2024); 1328208 (2024) https://doi.org/10.1117/12.3044481
Event: Second Advanced Imaging and Information Processing Conference (AIIP 2024), 2024, Xining, China

Abstract

Structured illumination microscopy is more suitable for super-resolution imaging of live cells and holds promising prospects in bioscience thanks to its fast imaging speed and low photodamage. However, in order to achieve fast, real-time and long-term imaging of live cells, further enhancements in imaging speed and reductions in photodamage are necessary. In this work, we optimize from both algorithm and hardware aspects. First, a Fourier ptychographic SIM algorithm (FP-SIM) using only three patterns is proposed to reduce the number of frames required for reconstruction. Second, we use CUDA to design appropriate parallel methods on hardware to accelerate the algorithm. We demonstrate that GPU improves the speed by nearly 200-fold in the most time-consuming iteration part of the algorithm. At 512×512 pixels, real-time super-resolution microscopy imaging at approximately 16Hz can be achieved. Faster imaging speed and less photodamage enable our method to provide a promising tool for life science research and biomedical measurement.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Jixiang Zhou, Jiaming Qian, and Yuzhen Zhang "Real-time structured illumination microscopy based on Fourier ptychographic", Proc. SPIE 13282, Second Advanced Imaging and Information Processing Conference (AIIP 2024), 1328208 (26 September 2024); https://doi.org/10.1117/12.3044481

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