Large-DOF ultraviolet photoacoustic microscopy using liquid crystal-based diffractive optical element

Yue Chen; Wei Song

doi:10.1117/12.3057321

10 January 2025 Large-DOF ultraviolet photoacoustic microscopy using liquid crystal-based diffractive optical element

Yue Chen, Wei Song

Author Affiliations +

Proceedings Volume 13507, Seventeenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2024); 135070N (2025) https://doi.org/10.1117/12.3057321
Event: Seventeenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2024), 2024, Shaya, China

Abstract

As strong optical absorption of DNA/RNA to Ultraviolet (UV) light, UV-Photoacoustic Microscopy (UV-PAM) can highlight the cell nuclei of tissues at outstanding imaging contrast without introduction of exogenous contrast agents. However, a short Depth of Focus (DOF) in the traditional UV-PAM results in out-of-focus imaging artifacts when imaging unprocessed tissue specimens that are usually characterized by uneven surface topology, which possibly degrades the histopathological examinations. We propose the wavefront engineering of UV photoacoustic illumination using a liquid crystal-based diffractive optical element (LC-DOE) to precisely manipulate the phases of the incident UV beam, enabling UV-PAM to produce an enlarged DOF (~250 μm) while maintaining subcellular lateral resolution (~1.02 μm) within the extended depth range. The system demonstrates high-resolution volumetric image of a phantoms made from many tungsten filaments. We anticipate that, with the liquid crystal-based diffractive optical element, the UV-PAM accommodates to image the unprocessed tissue samples, and thus potentially assists for non-destructive and label-free histopathology assessments of various disorders.

(2025) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yue Chen and Wei Song "Large-DOF ultraviolet photoacoustic microscopy using liquid crystal-based diffractive optical element", Proc. SPIE 13507, Seventeenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2024), 135070N (10 January 2025); https://doi.org/10.1117/12.3057321

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