Non-contact measurement of zebrafish heartbeat guided by OCT imaging

Jian Zou; Zaifan Wang; Kaihong Chen; Zhifang Li

doi:10.1117/12.3057534

10 January 2025 Non-contact measurement of zebrafish heartbeat guided by OCT imaging

Jian Zou, Zaifan Wang, Kaihong Chen, Zhifang Li

Author Affiliations +

Proceedings Volume 13507, Seventeenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2024); 135070Q (2025) https://doi.org/10.1117/12.3057534
Event: Seventeenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2024), 2024, Shaya, China

Abstract

The heart is the first organ to develop in zebrafish, and its developmental process is similar to that of the human heart. Zebrafish are an important model for studying human heart disease, and the accurate quantification of zebrafish heartbeats is instrumental in elucidating the intricacies of cardiac function and the pathogenesis of heart diseases, thereby facilitating a comprehensive understanding of potential therapeutic avenues. However, traditional methods such as electrocardiograph make it difficult to measure the heart rate of zebrafish in water. In this study, Optical Coherence Tomography (OCT) is employed to obtain high-precision images of the zebrafish heart area. These images are processed with an optical flow estimation algorithm to capture the motion information of the heart structure. Subsequently, a linear motion trajectory analysis algorithm is applied to fit waveforms to the extracted motion trajectories, enabling accurate computation of the zebrafish's heartbeat. The study demonstrates that this integrated approach enables high-precision, non-invasive measurement of zebrafish heartbeats, which can be used to monitor the changes in the zebrafish heartbeat induced by different anesthetic formulations.

(2025) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Jian Zou, Zaifan Wang, Kaihong Chen, and Zhifang Li "Non-contact measurement of zebrafish heartbeat guided by OCT imaging", Proc. SPIE 13507, Seventeenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2024), 135070Q (10 January 2025); https://doi.org/10.1117/12.3057534

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KEYWORDS

Heart

Optical coherence tomography

Detection and tracking algorithms

Optical flow

Image processing

Optical tracking

Image filtering

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