Influence of metronidazole and some electron acceptors on the chlorin e6 photosensitized killing of Ehrlich carcinoma cells

V. Chekulayev; Igor Shevchuk; Virgo Th. Mihkelsoo; A. P. Kallikorm

doi:10.1117/12.60936

1 June 1992 Influence of metronidazole and some electron acceptors on the chlorin e₆ photosensitized killing of Ehrlich carcinoma cells

V. Chekulayev, Igor Shevchuk, Virgo Th. Mihkelsoo, A. P. Kallikorm

Author Affiliations +

Proceedings Volume 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy; (1992) https://doi.org/10.1117/12.60936
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

A decrease in the effectiveness of photosensitized killing of neoplasm cells was observed in the presence of chlorin-e₆ at a reduced concentration of oxygen. But when metronidazole (MZ) was injected in vitro as well as in vivo, a significant increase in the photosensitized killing of Ehrlich carcinoma cells by chlorin-e₆ was observed. Moreover, contrary to the hematoporphyrin derivative (HpD), MZ increases the effectiveness of photodynamic therapy (PDT) by using chlorin-e₆ not only in the hypoxic but also in the aerobic conditions. The interaction between MZ and the excited photosensitizer may account for an increased phototoxicity of chlorin-e₆. The formation of cytotoxic nitroimidazole radicals as a result of photochemical processes of type 1 is discussed. This property of the photosensitizer may be successfully used in working out a method of potentiating PDT in combination not only with nitroimidazoles, but also with other electron acceptor compounds (EACp), e.g., quinone antitumor antibiotics.

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V. Chekulayev, Igor Shevchuk, Virgo Th. Mihkelsoo, and A. P. Kallikorm "Influence of metronidazole and some electron acceptors on the chlorin e₆ photosensitized killing of Ehrlich carcinoma cells", Proc. SPIE 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy, (1 June 1992); https://doi.org/10.1117/12.60936

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