Investigation of a near-infrared fiber optic immunosensor

Maryam Izadi Daneshvar; Guillermo A. Casay; Malgorzata Lipowska; Gabor Patonay; Lucjan Strekowski

doi:10.1117/12.170650

10 March 1994 Investigation of a near-infrared fiber optic immunosensor

Maryam Izadi Daneshvar, Guillermo A. Casay, Malgorzata Lipowska, Gabor Patonay, Lucjan Strekowski

Proceedings Volume 2068, Chemical, Biochemical, and Environmental Fiber Sensors V; (1994) https://doi.org/10.1117/12.170650
Event: Optical Tools for Manufacturing and Advanced Automation, 1993, Boston, MA, United States

Abstract

The designs of NIR optical fiber immunosensors for the detection of biomolecules are discussed. The use of fiber optics combined with laser-excited fluorescence detection ((lambda) _max equals 780 nm) and immunochemical techniques has provided the essential components for developing simpler and more practical, sensitive and selective immunosensors. The antibody is immobilized on the distal end of a surface-activated polymethyl methylacrylate sensor. As the probe is placed in different concentrations of antigenic substance, the antibody reacts with its corresponding antigen through sandwich and/or competitive immunoassays. The concentrations varied from 10 - 100 ng/ml. The near-infrared dye labeled antigen - antibody complex is excited and the emitted fluorescence is collected with a silicon photodiode detector equipped with an 820-nm bandpass filter. In order to determine various factors influencing the immunosensor's performance, the fluorescence intensity responses are obtained under a variety of conditions. The sensor response depending on the type of surface-activating reagent, surface activation period, incubation time, and other measurement conditions also are discussed.

Citation Download Citation

Maryam Izadi Daneshvar, Guillermo A. Casay, Malgorzata Lipowska, Gabor Patonay, and Lucjan Strekowski "Investigation of a near-infrared fiber optic immunosensor", Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); https://doi.org/10.1117/12.170650

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