Interaction of a nonspecific maize phospholipid transfer protein with unilamellar phospholipid vesicles: an infrared spectroscopy study

M. Subirade; Dominique Marion; Michel Pezolet

doi:10.1117/12.166603

31 January 1994 Interaction of a nonspecific maize phospholipid transfer protein with unilamellar phospholipid vesicles: an infrared spectroscopy study

M. Subirade, Dominique Marion, Michel Pezolet

Author Affiliations +

Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166603
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

The effect of a basic phospholipid transfer protein (PLTP) from maize seedings on unilamellar dimyristoyl-phosphatidylglycerol (DMPG) and dimyristoylphosphatidylcholine (DMPC) vesicles has been studied by Fourier transform infrared spectroscopy. The results show that the addition of PLTP affects the thermotropic behavior of large unilamellar vesicles of DMPG by increasing the temperature of the lipid gel-to-fluid phase transition by about 8 degree(s)C. Furthermore, the amide I region indicates that the protein undergoes a conformational change when it interacts with DMPG. On the other hand, PLTP does not interact with DMPC since pure DMPC and DMPC-PLTP complexes exhibit exactly the same temperature behavior. These results indicate that the presence of charges is necessary for the binding of the PLTP to phospholipids.

Citation Download Citation

M. Subirade, Dominique Marion, and Michel Pezolet "Interaction of a nonspecific maize phospholipid transfer protein with unilamellar phospholipid vesicles: an infrared spectroscopy study", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166603

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