Biodiagnostics with multiplex dyes

Stefan Seeger; Jutta Arden-Jacob; Gerhard Deltau; Karl-Heinz Drexhage; Kyung-Tae Han; Malte Koellner; Ralph Mueller; Markus Sauer; Juergen M. Wolfrum

doi:10.1117/12.180774

21 July 1994 Biodiagnostics with multiplex dyes

Stefan Seeger, Jutta Arden-Jacob, Gerhard Deltau, Karl-Heinz Drexhage, Kyung-Tae Han, Malte Koellner, Ralph Mueller, Markus Sauer, Juergen M. Wolfrum

Author Affiliations +

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180774
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A new bioanalytical concept that uses the characteristic fluorescence lifetimes of new multiplex dyes as an identification parameter is presented. Several dyes can be distinguished at the same wavelength by recognition of the fluorescence lifetimes. It is shown that the identification can be achieved in a capillary gel electrophoresis system in a few milliseconds with a misclassification rate of 10^-4. As an example the simultaneous detection of two antibodies at one wavelength is presented.

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Stefan Seeger, Jutta Arden-Jacob, Gerhard Deltau, Karl-Heinz Drexhage, Kyung-Tae Han, Malte Koellner, Ralph Mueller, Markus Sauer, and Juergen M. Wolfrum "Biodiagnostics with multiplex dyes", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180774

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